OCR GCSE Chemistry A J248
Chemical Reactions
Elements, Compounds and Mixtures
Global Challenges
Monitoring and Controlling Chemical Reactions
Particles
Predicting and Identifying Reactions
Elements, Compounds and Mixtures
Paper and thin layer chromatography
- 3 things to know
- Paper 1
Overview
How a paper chromatogram is set up with a pencil baseline above the solvent, what a thin layer plate is made of and its advantage over paper, choosing a solvent, revealing colourless spots with a locating agent or ultraviolet light, the stationary and mobile phases in paper and thin layer chromatography, and why substances separate.
What you need to know
Answer each one before you reveal it.
Describe how a paper chromatogram is set up.
Why is the baseline drawn in pencil rather than in ink?
Questions from this subtopic
Each one with the model answer underneath.
- 1
Describe how a paper chromatogram is set up.
Model answer
A baseline is drawn near the bottom of the paper, spots of the mixture are placed on it, and the paper is stood in solvent so that the solvent level is below the baseline and rises through the paper.
- 2
Why is the baseline drawn in pencil rather than in ink?
Model answer
Because pencil is insoluble in the solvent, so it will not travel up the paper or add spots of its own.
- 3
Why must the solvent level start below the baseline?
Model answer
So the spots dissolve into the solvent as it rises past them, instead of washing straight off the paper into the solvent below.
- 4
What is the plate made from in thin layer chromatography?
Model answer
It is a sheet of glass or plastic coated with a thin, even layer of silica or alumina.
- 5
Give an advantage of thin layer chromatography over paper chromatography.
Model answer
It is faster and gives sharper separation, because the coating is finer and more uniform than paper fibres.
- 6
When would a non-aqueous solvent such as ethanol be chosen instead of water?
Model answer
When the substances being separated do not dissolve in water, so they need an organic solvent to carry them.
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