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Chemical Analysis

Chromatography

  • 3 things to know
  • Chemistry Paper 2

Overview

The stationary and mobile phases, what decides how far a substance travels, how Rf values are defined and calculated, reading purity from a chromatogram, and why the start line is pencil and the solvent starts below it.

What you need to know

Answer each one before you reveal it.

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Key term

What are the two phases involved in any form of chromatography?

Evaluation

What decides how far a substance travels during chromatography?

Key term

How is the Rf value of a compound defined?

Questions from this subtopic

Each one with the model answer underneath.

  1. 1

    What are the two phases involved in any form of chromatography?

    Model answer

    A stationary phase, which stays still, and a mobile phase, which moves through it.

  2. 2

    What can chromatography do for a mixture?

    Model answer

    It separates the substances in the mixture and gives information that helps to identify them.

  3. 3

    What decides how far a substance travels during chromatography?

    Model answer

    How that substance is distributed between the mobile phase and the stationary phase.

  4. 4

    Explain why one substance in a mixture travels further up the paper than another.

    Model answer

    It is held less strongly by the stationary phase and carried more readily by the moving solvent.

  5. 5

    How is the Rf value of a compound defined?

    Model answer

    It is the distance moved by the compound, measured to the centre of its spot, divided by the distance moved by the solvent.

  6. 6

    A spot moves 3.6 cm from the origin while the solvent moves 8.0 cm. Calculate its Rf value.

    Model answer

    Rf is 3.6 ÷ 8.0, which is 0.45.

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