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Cells, Viruses and Reproduction of Living Things

Investigating pollen tube growth

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  • Papers 1, 2 and 3

Overview

Recall questions on investigating how sucrose concentration affects pollen tube growth: preparing the solutions and medium, why too little or too much sucrose stops germination, the variables to control, and measuring tube length or germination percentage.

What you need to know

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Key term

Why is pH a controlled variable in the pollen germination investigation?

Questions from this subtopic

Each one with the model answer underneath.

  1. 1

    Why is pH a controlled variable in the pollen germination investigation?

    Model answer

    pH affects enzyme activity; variations could alter germination rates independently of sucrose concentration.

  2. 2

    Why should the volume of sucrose solution be kept constant in the pollen germination practical?

    Model answer

    To ensure pollen grains are exposed to consistent conditions and that differences in results are due to sucrose concentration, not volume.

  3. 3

    Why is using pollen from the same source important in the germination investigation?

    Model answer

    Different pollen sources may have different germination rates, so using the same source eliminates this as a variable.

  4. 4

    What happens to pollen grains if the sucrose concentration in the germination medium is too low?

    Model answer

    The pollen grains burst due to osmotic uptake of water.

  5. 5

    What happens to pollen grains if the sucrose concentration is too high?

    Model answer

    The pollen grains undergo plasmolysis.

  6. 6

    Why is there an optimum sucrose concentration for pollen germination?

    Model answer

    Concentrations too low cause bursting from excessive water uptake, while concentrations too high cause plasmolysis; the optimum balances osmotic conditions for successful germination.

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