Evaluation
Edexcel A-Level Biology B 9BI0
Biological Molecules
Topic overviewMonosaccharides, disaccharides and polysaccharidesGlycosidic bonds and condensation reactionsStarch, glycogen and cellulose structureTriglycerides and phospholipidsSaturated and unsaturated fats and lipid propertiesAmino acid structure and the peptide bondPrimary, secondary, tertiary and quaternary structureFibrous and globular proteinsThe structure of DNADNA replication and gene mutationsThe structure of RNATranscription and translationEnzymes as globular proteinsFactors affecting enzyme activityMeasuring the rate of enzyme activityUnits, uncertainty and experimental errorEnzyme inhibitionInorganic ionsThe properties of water
Cells, Viruses and Reproduction of Living Things
Topic overviewCell theory and eukaryotic cell structureProkaryotic cells and Gram stainingMicroscopy, magnification and resolutionSignificant figures, estimates and mathematical symbolsVirus structure and classificationThe lytic cycle and latencyTreating viral disease and the ethics of untested drugsThe cell cycle and its regulationMitosis and the behaviour of chromosomesMitosis in growth, repair and root tip squashesMeiosis, genetic variation and chromosome mutationsGametogenesis in mammalsFertilisation and early embryo developmentPollen and embryo sac formationDouble fertilisation in flowering plantsInvestigating pollen tube growth
Classification and Biodiversity
Exchange and Transport
Topic overviewSurface area to volume ratioThe fluid mosaic modelDiffusion, osmosis and facilitated diffusionActive transport, endocytosis and exocytosisGas exchange in insects, fish and mammalsDissecting the insect gas exchange systemGas exchange in flowering plantsThe structure of the heart and blood vesselsThe double circulatory systemThe cardiac cycleMyogenic stimulation and ECG tracesThe composition of bloodThe functions of blood and clottingAtherosclerosis and its risk factorsHaemoglobin and the Bohr effectOxygen dissociation curvesFormation of tissue fluidThe lymphatic systemXylem and phloem structureApoplastic and symplastic pathwaysThe cohesion-tension theoryMeasuring transpiration with a potometerTranslocation and the mass flow hypothesis
Energy for Biological Processes
Topic overviewAn overview of aerobic respirationGlycolysisThe link reaction and the Krebs cycleThe electron transport chainChemiosmosis and ATP yieldAnaerobic respiration and ATP yieldMeasuring respiration with a respirometerAbsorption and action spectraPhotosynthetic pigments and chromatographyChloroplast structure and the light-dependent stageThe Calvin cycleLimiting factors in photosynthesis
Microbiology and Pathogens
Topic overviewCulturing microorganisms and aseptic techniqueMeasuring bacterial growthThe bacterial growth curveBacteria as pathogensThe action of antibioticsAntibiotic resistanceOther pathogenic agentsControlling endemic diseasePhagocytosis and the humoral responseThe cell-mediated immune responseMemory cells and the secondary responseNatural and artificial immunityVaccination and herd immunity
Modern Genetics
Origins of Genetic Variation
Topic overviewMutation, meiosis and random fertilisationGenotype, phenotype and monohybrid inheritanceDihybrid crosses and pedigree diagramsAutosomal linkage and sex linkageThe chi-squared test in geneticsSelection pressures and allele frequencyGenetic drift and the founder effectThe Hardy-Weinberg equation
Control Systems
Topic overviewHomeostasis and negative feedbackChemical control in mammalsPlant growth substancesAuxins, cytokinins and apical dominancePhytochrome and photomorphogenesisThe organisation of the nervous systemBrain regions and divisions of the peripheral nervous systemThe autonomic nervous systemResting potential and the action potentialPropagation and saltatory conductionSynaptic transmissionEffects of drugs on the nervous systemThe structure of the retina and rhodopsinRods, cones and visual acuityControl of heart rateKidney structure and ultrafiltrationSelective reabsorption and the loop of HenleADH and osmoregulationThermoregulation in endotherms
Ecosystems
Topic overviewEcosystems, habitats and nichesPyramids of numbers, biomass and energyEcological techniques for measuring distributionChoosing the right ecological techniquePresenting data in tables and graphsStatistical tests in ecologyProductivity and energy transfer efficiencyDecomposers and nutrient cyclingSuccessionBiotic and abiotic factors in population changeHuman influences on ecosystemsSustainability, resource management and peer review
Microbiology and Pathogens
Measuring bacterial growth
- 2 things to know
- Papers 1 and 3
Overview
Recall questions on total and viable counts, dilution plating, turbidity, and the haemocytometer.
What you need to know
Answer each one before you reveal it.
Your recallNot started
Key term
How is optical density measured when monitoring bacterial growth?
Questions from this subtopic
Each one with the model answer underneath.
- 1
What is a limitation of direct cell counting using a haemocytometer?
Model answer
It includes both living and dead cells in the count, so does not distinguish viable cells.
- 2
How does a haemocytometer enable direct cell counting?
Model answer
It has a counting chamber of known volume, allowing cell concentration to be determined by counting cells in a defined space.
- 3
Describe the principle of dilution plating for counting bacteria.
Model answer
A culture is serially diluted, then known volumes are spread onto agar plates to count colony-forming units.
- 4
Why does dilution plating count only viable cells?
Model answer
Because each colony arises from one living cell or group of cells; dead cells cannot form colonies.
- 5
How is dry mass measurement of bacterial growth carried out?
Model answer
Culture samples are centrifuged, the pellet is dried, and the dry mass is measured.
- 6
How does turbidity measurement indicate bacterial growth?
Model answer
A colorimeter or spectrophotometer measures light absorbance, which increases as cell density increases.
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