OCR A-Level Biology H420
Cell Structure
Biological Molecules
Nucleotides and Nucleic Acids
Enzymes
Biological Membranes
Cell Division, Cell Diversity and Cellular Organisation
Exchange Surfaces
Transport in Animals
Transport in Plants
Communicable Diseases, Disease Prevention and the Immune System
Biodiversity
Classification and Evolution
Communication and Homeostasis
Excretion as an Example of Homeostatic Control
Neuronal Communication
Hormonal Communication
Plant and Animal Responses
Photosynthesis
Respiration
Cellular Control
Patterns of Inheritance
Manipulating Genomes
Cloning and Biotechnology
Ecosystems
Populations and Sustainability
Practical Skills and Mathematical Requirements
Cloning and Biotechnology
- 10 subtopics
- 19 things to know
- Components 02 and 03
What Cloning and Biotechnology covers
Cloning and biotechnology is the fourth section of Module 6. It covers natural and artificial plant cloning, micropropagation, embryo twinning and nuclear transfer in animals, the arguments for and against each, microorganisms in food and medicine, aseptic technique and fermentation, and immobilised enzymes.
Where it is assessed
Components 02 and 03
Module 6 is examined on Biological Diversity, then the unified paper.
Check the OCR specification ↗The 10 subtopics
Each one has its own page.
- Natural plant cloning and taking cuttingsRunners, rhizomes, bulbs, corms, tubers and suckers as natural clones, and the steps in taking a cutting and getting it to root.Open →
- MicropropagationThe explant and why it is surface sterilised, the hormones in the nutrient agar, the callus that forms, and the hardening off that ends the process.Open →
- Advantages and disadvantages of plant cloningRapid mass production, preserved traits, disease-free stock and growing out of season, set against reduced genetic diversity, cost and the skill required.Open →
- Animal cloning: embryo twinning and nuclear transferHow identical twins arise naturally, the splitting of an early embryo, and the enucleation and nuclear transfer steps of somatic cell nuclear transfer.Open →
- Advantages and disadvantages of animal cloningElite genetics, medical uses, conservation and research, set against low success rates, health problems, reduced longevity, cost and ethical objections.Open →
- Microorganisms in food productionWhy microorganisms suit industrial production, the speed, land use and waste substrates involved, mycoprotein from Fusarium, and the objections raised to microbial food.Open →
- Aseptic techniqueSterilising equipment and growth media, working near a flame, flaming bottle necks and inoculating loops, and how Petri dishes are handled and taped.Open →
- Fermentation and its conditionsBatch and continuous fermentation, and why temperature, pH, oxygen supply, nutrient concentration and agitation are monitored and controlled.Open →
- The microbial growth curveThe lag, exponential, stationary and death phases, the calculation of numbers after n generations, and the conditions that shape microbial growth.Open →
- Immobilised enzymesAdsorption, covalent bonding, entrapment and encapsulation, and the reusability, stability and continuous flow they allow, set against reduced activity, diffusion limits and cost.Open →
What you need to know
Answer each one before you reveal it.
Describe the microbial growth curve in closed culture.
Evaluate the use of immobilised enzymes.
What is a financial disadvantage of micropropagation?
What is the final step in SCNT before the clone develops?
How does land use for microbial food production compare to conventional farming?
What is aseptic technique?
What is a financial disadvantage of using immobilised enzymes?
What is a corm and how does it contribute to natural cloning?
What is tissue culture in plants?
What is the first step in artificial embryo twinning?
What is the first step in somatic cell nuclear transfer (SCNT)?
Define biotechnology.
What is a social barrier to microbial food production?
What is mycoprotein?
Why is nutrient concentration monitored during fermentation?
What are immobilised enzymes?
Why might immobilised enzymes have reduced activity compared to free enzymes?
Evaluate the cloning of animals.
Take Cloning and Biotechnology further
The same content, four other ways.
Commonly confused
Pairs that lose marks when they are blurred together.
Natural clone vs Artificial clone
Natural clone: a genetically identical organism produced without human intervention. Artificial clone: a genetically identical organism produced deliberately by a technique.
Reproductive cloning vs Therapeutic cloning
Reproductive cloning: producing a whole new organism genetically identical to another. Therapeutic cloning: producing cells or tissue for treatment rather than a whole organism.
Batch culture vs Continuous culture
Batch culture: a closed fermentation run that is harvested and restarted. Continuous culture: a fermentation with nutrients added and product removed while it runs.
Questions from this topic
Each one with the model answer underneath.
- 1
What is vegetative propagation in plants?
Model answer
A form of natural cloning where new individuals arise from vegetative structures (such as stems, roots, or leaves) rather than from seeds.
- 2
What are runners or stolons in the context of vegetative propagation?
Model answer
Horizontal stems that grow along the soil surface and produce new plants at nodes, as seen in strawberries.
- 3
What are rhizomes and how do they enable vegetative propagation?
Model answer
Underground horizontal stems that grow beneath the soil surface and produce new shoots and roots at intervals, allowing the plant to spread and form clones.
- 4
How do bulbs function as structures for vegetative propagation?
Model answer
Bulbs are underground storage organs consisting of a short stem surrounded by fleshy leaf bases; they produce daughter bulbs that can separate and grow into new plants.
- 5
What is a corm and how does it contribute to natural cloning?
Model answer
A corm is a swollen underground stem base that stores nutrients; it produces new corms (cormels) that develop into genetically identical plants.
- 6
How do tubers enable vegetative propagation?
Model answer
Tubers are swollen underground stems or roots that store nutrients and have buds (eyes) that can sprout to produce new genetically identical plants.
What is inside Levo
Six ways to work the same material.
Cloning and Biotechnology revision questions
- What does Cloning and Biotechnology cover in OCR A-Level Biology?
- 10 subtopics: natural plant cloning and taking cuttings, micropropagation, advantages and disadvantages of plant cloning, animal cloning: embryo twinning and nuclear transfer, advantages and disadvantages of animal cloning, microorganisms in food production, aseptic technique, fermentation and its conditions, the microbial growth curve and immobilised enzymes. Each one has its own page with questions and model answers.
- How is Cloning and Biotechnology organised?
- By the OCR H420 specification, subtopic by subtopic, so it matches the order you are taught it in.
Every question in Cloning and Biotechnology, on a schedule.
Attempt a question from memory, write the correct version, then attempt it again. Levo records the jump between your first attempt and your last, and brings each question back on the day you are due to forget it.
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