AQA GCSE Biology 8461
Bioenergetics
Cell Biology
Ecology
Homeostasis and Response
Infection and Response
Inheritance, Variation and Evolution
Organisation
Cell Biology
Growing bacteria and aseptic technique
- 3 things to know
- Paper 1
Overview
Binary fission and how fast bacteria divide, growing bacteria in nutrient broth or as colonies on agar plates, why cultures must be uncontaminated, and aseptic technique, from sterilising dishes and flaming the inoculating loop to taping lids, storing plates upside down and incubating at 25 °C.
What you need to know
Answer each one before you reveal it.
By what process do bacteria reproduce?
How often can bacteria divide in ideal conditions?
Questions from this subtopic
Each one with the model answer underneath.
- 1
By what process do bacteria reproduce?
Model answer
Simple cell division, called binary fission.
- 2
How often can bacteria divide in ideal conditions?
Model answer
As often as once every 20 minutes.
- 3
What two conditions do bacteria need in order to divide as rapidly as possible?
Model answer
Enough nutrients and a suitable temperature.
- 4
Suggest why bacteria in a sealed culture eventually stop dividing so quickly.
Model answer
The nutrients in the culture are used up, so growth slows.
- 5
Name two ways bacteria can be grown in a laboratory.
Model answer
In a nutrient broth solution, or as colonies on an agar gel plate.
- 6
What is a bacterial colony?
Model answer
A visible patch of many bacteria on an agar plate, all grown by division from one original cell.
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