levo

Cell Biology

Growing bacteria and aseptic technique

  • 3 things to know
  • Paper 1

Overview

Binary fission and how fast bacteria divide, growing bacteria in nutrient broth or as colonies on agar plates, why cultures must be uncontaminated, and aseptic technique, from sterilising dishes and flaming the inoculating loop to taping lids, storing plates upside down and incubating at 25 °C.

What you need to know

Answer each one before you reveal it.

Your recallNot started
Key term

Explain why a disinfectant test needs an uncontaminated culture and how aseptic technique, sterilising equipment and flaming the loop prevent contamination.

Process

By what process do bacteria reproduce?

Key term

How often can bacteria divide in ideal conditions?

Questions from this subtopic

Each one with the model answer underneath.

  1. 1

    By what process do bacteria reproduce?

    Model answer

    Simple cell division, called binary fission.

  2. 2

    How often can bacteria divide in ideal conditions?

    Model answer

    As often as once every 20 minutes.

  3. 3

    What two conditions do bacteria need in order to divide as rapidly as possible?

    Model answer

    Enough nutrients and a suitable temperature.

  4. 4

    Suggest why bacteria in a sealed culture eventually stop dividing so quickly.

    Model answer

    The nutrients in the culture are used up, so growth slows.

  5. 5

    Name two ways bacteria can be grown in a laboratory.

    Model answer

    In a nutrient broth solution, or as colonies on an agar gel plate.

  6. 6

    What is a bacterial colony?

    Model answer

    A visible patch of many bacteria on an agar plate, all grown by division from one original cell.

Other ways to work this

The same content, four other ways.

What is inside Levo

Six ways to work the same material.

1Closed book
2Open book
3Closed book

The 3-step method, run for you

Answer cold, learn it, then prove it stuck. Levo tracks the jump between your first attempt and your last.

Bioenergetics
Cell Biology
Ecology
Homeostasis and Response

Cram a whole paper in one sitting

Sort every topic into what you know and what you do not, then drill only the weak ones.

A set.
singledoublefull

Blank Blitz makes facts automatic

Rapid timed gap-fills on the terms and numbers you need to recall without thinking.

AA sugar, a phosphate group and one of four bases.
BThe order of the bases in the DNA.
C10 amino acids, because each amino acid is coded for by three bases.

Build It gets the exam shape right

Assemble a full-mark answer from its marking points, in the order an examiner wants them.

Habitat means the place in which an organism lives.
TrueFalse

Test It catches misconceptions

Spot the fault in a statement. The traps are the mistakes students actually make.

721941163128510

Brain Breaks between sessions

Short focus games to reset attention, so the next set lands better than the last.

Every question in Cell Biology, on a schedule.

Attempt a question from memory, write the correct version, then attempt it again. Levo records the jump between your first attempt and your last, and brings each question back on the day you are due to forget it.

Or £46 a year, about £3.83 a month. Cancel anytime.