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Cell Biology

Microscopy

  • 3 things to know
  • Paper 1

Overview

Magnification and resolution, how electron microscopes compare with light microscopes and what they revealed about sub-cellular structures, and calculating magnification, image size and real size, including conversion to micrometres and standard form.

What you need to know

Answer each one before you reveal it.

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Key term

What is meant by the magnification of a microscope?

Key term

What is meant by the resolution of a microscope?

Key term

Which property limits how much detail can be seen, magnification or resolution?

Questions from this subtopic

Each one with the model answer underneath.

  1. 1

    What is meant by the magnification of a microscope?

    Model answer

    How many times larger the image is than the real object.

  2. 2

    What is meant by the resolution of a microscope?

    Model answer

    The smallest distance between two points that can still be seen as separate, which sets how much detail is visible.

  3. 3

    Which property limits how much detail can be seen, magnification or resolution?

    Model answer

    Resolution, because magnifying further only makes a blurred image bigger.

  4. 4

    Compare an electron microscope with a light microscope.

    Model answer

    An electron microscope has much higher magnification and much higher resolving power.

  5. 5

    Explain why an electron microscope can show structures a light microscope cannot.

    Model answer

    Its higher resolving power lets two very close points be seen separately, so cells are shown in much finer detail.

  6. 6

    How has the development of microscopes changed what biologists know about cells?

    Model answer

    Better instruments have revealed more sub-cellular structures, so their functions could be worked out.

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