OCR A-Level Biology B H422
The Developing Cell: Cell Division and Cell Differentiation
The Developing Individual: Meiosis, Growth and Development
The Development of Species: Evolution and Classification
Cells and Microscopy
Water and Its Importance in Plants and Animals
Proteins and Enzymes
Nucleic Acids
Patterns of Inheritance
Population Genetics and Epigenetics
Gene Technologies
The Principles and Importance of Homeostasis
The Hormonal Control of Blood Glucose and the Management of Diabetes
Kidney Functions and Malfunctions
The Cellular Basis of Cancer and Treatment
Respiratory Diseases and Treatment
Cellular Respiration
Metabolism and Exercise
Pathogenic Microorganisms
The Immune System
Controlling Communicable Diseases
Photosynthesis, Food Production and Management of the Environment
The Impact of Population Increase
Planning and Implementing Practical Work
Analysing and Evaluating Practical Results
Practical Skills, Apparatus and Techniques
Fertility and Assisted Reproduction
The Effects of Ageing on the Reproductive System
Plant Reproduction
The Nervous System and the Identification and Consequences of Damage
Monitoring Visual Function
The Effect of Ageing on the Nervous System
The Heart and Monitoring Heart Function
Transport Systems in Mammals
Gas Exchange in Mammals and Plants
Cells and Microscopy
Blood smears, staining and microscope drawings
- 3 things to know
- Components 01, 02 and 03
Overview
Recall questions on preparing and drying a blood smear safely, differential staining with Leishman's stain, recognising erythrocytes, neutrophils, lymphocytes, monocytes and platelets in a stained smear, and the conventions for biological drawings, plan drawings and high-power drawings.
What you need to know
Answer each one before you reveal it.
Why is a blood smear allowed to air dry before it is stained?
State two safety precautions taken when preparing blood smears in a school laboratory.
Questions from this subtopic
Each one with the model answer underneath.
- 1
Describe how a blood smear is prepared on a slide.
Model answer
A small drop of blood is placed near one end of a clean slide, a second slide is held against it at about 30 to 45 degrees and drawn back into the drop so the blood spreads along the edge, then pushed steadily forward to leave a thin even film that is allowed to air dry.
- 2
Why must a blood smear be a thin film?
Model answer
So that the cells lie in a single layer and can be seen and identified individually instead of overlapping one another.
- 3
Why is a blood smear allowed to air dry before it is stained?
Model answer
Drying makes the cells adhere to the slide so that they are not washed off during staining and rinsing.
- 4
State two safety precautions taken when preparing blood smears in a school laboratory.
Model answer
Only supplied, screened mammalian blood is used rather than the students' own blood, and gloves are worn with all slides and glassware disinfected and disposed of as clinical waste.
- 5
What is meant by differential staining?
Model answer
It is the use of a stain, or a mixture of stains, that colours different cell types or different cell components differently so that they can be told apart.
- 6
Which stain is used to identify leucocytes in a blood smear?
Model answer
Leishman's stain.
Other ways to work this
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