Key term
OCR A-Level Biology B H422
The Developing Cell: Cell Division and Cell Differentiation
The Developing Individual: Meiosis, Growth and Development
The Development of Species: Evolution and Classification
Cells and Microscopy
Topic overviewMicroscopy and the cell theoryBlood smears, staining and microscope drawingsBlood cells and their structureMagnification and measuring cellsHaemocytometer cell counts and dilutionsFlow cytometry in blood analysisUltrastructure of the animal cellPlant and prokaryotic cellsMembrane lipids and the fluid mosaic modelMovement of molecules across membranesThe roles of membranes and the secretion of proteins
Water and Its Importance in Plants and Animals
Proteins and Enzymes
Nucleic Acids
Patterns of Inheritance
Population Genetics and Epigenetics
Gene Technologies
The Principles and Importance of Homeostasis
The Hormonal Control of Blood Glucose and the Management of Diabetes
Kidney Functions and Malfunctions
Topic overviewExcretion and the gross structure of the kidneyThe nephron: ultrafiltration and selective reabsorptionInvestigating the composition of urine, plasma and filtrateOsmoregulation and the action of ADHThe kidney as an endocrine gland and kidney failureDialysis, transplantation and the future of transplant surgery
The Cellular Basis of Cancer and Treatment
Respiratory Diseases and Treatment
Cellular Respiration
Metabolism and Exercise
Topic overviewThe consequences of exercise on the bodyAerobic fitness and VO₂ maxTraining, F.I.T.T. factors and investigating fitnessEnhancing athletic performanceHaemoglobin and the transport of oxygenOxygen dissociation curves of different respiratory pigmentsOxygen deficit and oxygen debtThe structure of skeletal muscleThe sliding filament theory of muscle contraction
Pathogenic Microorganisms
The Immune System
Controlling Communicable Diseases
Photosynthesis, Food Production and Management of the Environment
Topic overviewChloroplast ultrastructure and photosynthetic pigmentsThe light-dependent stage of photosynthesisThe Calvin cycleInvestigating the factors affecting photosynthesisThe Hill reaction and DCPIPUsing TP and GP to build carbohydrates, lipids and amino acidsThe compensation point and crop productionMicroorganisms, the nitrogen cycle and biosensorsBiomass transfer and efficiency in food productionRuminants in the human food chainFarms as ecosystems and the conflict with conservationDeflected succession and measuring biodiversity
The Impact of Population Increase
Planning and Implementing Practical Work
Analysing and Evaluating Practical Results
Practical Skills, Apparatus and Techniques
Topic overviewInvestigative approaches and working safelyFollowing instructions, recording and presenting dataResearch, referencing and processing data with softwareMeasuring mass, volume, time, temperature, length and pHColorimeters and potometersLaboratory glassware and serial dilutionsLight microscopy, graticules and biological drawingQualitative reagents for biological moleculesChromatography and electrophoresisAseptic technique and dissectionWhole-organism responses, fieldwork sampling and data logging
Fertility and Assisted Reproduction
The Effects of Ageing on the Reproductive System
Plant Reproduction
The Nervous System and the Identification and Consequences of Damage
Topic overviewOrganisation of the nervous system and nervous tissueThe structure and function of the human brainThe structure of motor, sensory and relay neuronesResting potential and the action potentialSynapses and the integration of responsesReflexes, reaction times and their investigationBrain scans and the consequences of nervous system damageDrugs, brain function and dependency
Monitoring Visual Function
The Effect of Ageing on the Nervous System
The Heart and Monitoring Heart Function
Transport Systems in Mammals
Gas Exchange in Mammals and Plants
Proteins and Enzymes
Enzyme action and the rate of reaction
- 3 things to know
- Components 01, 02 and 03
Overview
Recall questions on enzyme specificity and the induced fit model, activation energy, the effects of temperature, Q₁₀, pH, substrate and enzyme concentration, competitive and non-competitive inhibitors, and measuring initial rates of catalase activity with a gas syringe.
What you need to know
Answer each one before you reveal it.
Your recallNot started
Key term
What is the effect of increasing temperature on the rate of an enzyme-catalysed reaction, and why?
Key term
Describe how the effect of substrate concentration on the rate of catalase activity can be investigated.
Questions from this subtopic
Each one with the model answer underneath.
- 1
What is an enzyme?
Model answer
An enzyme is a globular protein that acts as a biological catalyst, speeding up a specific metabolic reaction without being used up or permanently changed by it.
- 2
How does the tertiary structure of an enzyme give it its specificity?
Model answer
The folding of the chain creates an active site whose shape and pattern of charges are complementary to one particular substrate, so no other molecule can bind and be converted.
- 3
What does the induced fit model of enzyme action describe?
Model answer
The active site is not initially an exact match for the substrate; as the substrate binds, the enzyme changes shape slightly so the site moulds closely around it, which puts strain on the substrate's bonds.
- 4
What is an enzyme–substrate complex?
Model answer
It is the temporary structure formed when a substrate binds to the active site of an enzyme, held in place by weak interactions between them.
- 5
What is meant by activation energy?
Model answer
It is the minimum energy that colliding reactant molecules must have before they will react.
- 6
In what two ways does an enzyme lower the activation energy of a reaction?
Model answer
It holds two substrates together in exactly the right orientation for the reaction to occur, and by moulding around the substrate it strains and weakens the bonds that need to be broken.
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