Which feature of ATP synthetase allows it to use a proton gradient?

It contains a channel through the membrane, and this is the only route by which protons can cross an otherwise impermeable membrane.

State the reaction catalysed by ATP synthetase.

ADP combines with an inorganic phosphate group to give ATP and water.

Define oxidative phosphorylation.

The synthesis of ATP using energy released as electrons pass along the electron transport chain in respiration, with oxygen as the final electron acceptor.

Eduqas A-Level Biology A400QS

Biological Molecules

Topic overview Inorganic ions and the properties of water Monosaccharides and disaccharides Polysaccharides, storage and structure Lipids, triglycerides and phospholipids Amino acids and protein structure Condensation, hydrolysis and the food tests

Cell Structure and Organisation

Topic overview The nucleus and the endomembrane system Mitochondria, chloroplasts and other organelles Prokaryotic cells and viruses Cell theory, microscopy and measuring cells Comparing cells and the levels of organisation

Cell Membranes and Transport

Topic overview The fluid-mosaic model Factors affecting membrane permeability Diffusion and facilitated diffusion Osmosis and water potential Active transport and bulk transport Determining water potential in practice

Enzymes

Topic overview Enzymes as biological catalysts The active site and induced fit Temperature, pH and buffers Substrate and enzyme concentration Inhibition and immobilised enzymes

Nucleic Acids and Their Functions

Topic overview Nucleotides and the structure of ATP ATP as the energy currency of the cell The structure of DNA Comparing DNA and RNA Semi-conservative replication The genetic code Transcription Translation and the fate of the polypeptide Extracting DNA from living material

Importance of ATP

Topic overview ATP synthetase and the synthesis of ATP Chemiosmosis and the electrochemical gradient Proton pumps and the electron transport chain Membranes of mitochondria and chloroplasts compared Investigating dehydrogenase activity

Photosynthesis

Topic overview Chloroplast structure and the distribution of chloroplasts Photosynthetic pigments and chromatography Photosystems and light harvesting Non-cyclic and cyclic photophosphorylation The light-independent stage Limiting factors and the rate of photosynthesis Nitrogen, magnesium and plant growth

Respiration

Topic overview The need for respiration and glycolysis The link reaction and the Krebs cycle Reduced NAD, FAD and oxidative phosphorylation The energy budget of aerobic and anaerobic respiration Respiratory substrates and investigating respiration in yeast

Microbiology

Topic overview Bacterial shape and cell wall structure The Gram stain Culturing microorganisms in the laboratory Conditions for growth and aseptic technique Monitoring population growth and the milk investigation

Population Size and Ecosystems

Topic overview Population growth and its measurement The population growth curve and carrying capacity Density dependent and density independent factors Sampling abundance and distribution Ecosystems, habitats and communities Energy transfer and pyramids of biomass Succession Decomposition and the carbon cycle The nitrogen cycle and human intervention Eutrophication and the effects of drainage

Human Impact on the Environment

Topic overview Endangered species and extinction Conserving gene pools in the wild and in captivity Deforestation and overfishing Sustainability and informed decision making Planetary boundaries

Classification and Biodiversity

Topic overview The need for classification and the taxonomic hierarchy The three domain and five kingdom systems Characteristic features of the five kingdoms Assessing relatedness by physical features and biochemistry The species concept and the binomial system Biodiversity, adaptation and natural selection Measuring biodiversity in a habitat Genetic and molecular measures of biodiversity

Mitosis and Meiosis

Topic overview The cell cycle and interphase The stages of mitosis Cytokinesis and the significance of mitosis The stages of meiosis Comparing mitosis with meiosis, and seeing them on prepared slides

Sexual Reproduction in Humans

Topic overview The reproductive systems and the histology of testis and ovary Spermatogenesis and oogenesis Intercourse, fertilisation and implantation The menstrual cycle and its hormonal control The placenta, birth and lactation

Sexual Reproduction in Plants

Topic overview Flower structure and the contrast between wind and insect pollination The development of pollen grains and ovules Pollination and double fertilisation The formation and structure of seed and fruit Germination of Vicia faba and the action of gibberellin

Inheritance

Topic overview Alleles, monohybrid inheritance and codominance Dihybrid inheritance and linkage The chi squared test and testing genetic ratios Sex linkage, haemophilia and Duchenne muscular dystrophy Mutation, mutagens and epigenetics

Variation and Evolution

Topic overview Genetic and environmental sources of variation Investigating continuous variation and the t test Competition and selective agencies Gene pools, genetic drift and selection The Hardy-Weinberg principle Isolation, speciation and Darwin's theory of evolution

Application of Reproduction and Genetics

Topic overview The Human Genome Project, other sequenced genomes and genomics in healthcare Screening embryos, stem cells and the ethical issues Genetic fingerprinting, PCR and electrophoresis in forensic work Recombinant DNA and the production of human insulin Genetically modified crops and gene therapy

Adaptations for Gas Exchange

Topic overview Surface area to volume ratio and gas exchange in small organisms Specialised respiratory surfaces and the need for ventilation Gills and counter current flow in bony fish The human breathing system and its histology Ventilation and gas exchange in humans The insect tracheal system The angiosperm leaf, stomata and gas exchange

Adaptations for Transport

Topic overview Vascular systems compared across animal groups The mammalian heart and blood vessels The cardiac cycle and its control Blood, haemoglobin and dissociation curves The Bohr effect, chloride shift and tissue fluid The root and the uptake of water The endodermis and the entry to the xylem Xylem, transpiration and cohesion-tension The potometer and xerophytes and hydrophytes Phloem and translocation

Adaptations for Nutrition

Topic overview Modes of nutrition Saprotrophic nutrition Holozoic nutrition and the unicellular gut From a sac-like gut to a tube gut The human gut and the effect of diet Herbivores, carnivores and parasites

Homeostasis and the Kidney

Topic overview Homeostasis and feedback The structure of the kidney and the nephron Ultrafiltration and selective reabsorption Water regulation and antidiuretic hormone Excretory products, adaptation and kidney failure

The Nervous System

Topic overview The components of a nervous response The spinal cord and spinal nerves The reflex arc and nerve nets The structure of a motor neurone The nerve impulse Oscilloscope traces and conduction speed The synapse and synaptic transmission Chemicals affecting transmission

Immunology and Disease: Disease and Its TransmissionImmunology

Topic overview The language of infection and disease The human body as a host Cholera and tuberculosis Smallpox, influenza and viral pathogenicity Malaria and its transmission

Immunology and Disease: AntibioticsImmunology

Topic overview Bacteriostatic and bactericidal antibiotics Broad and narrow spectrum antibiotics Penicillin and the bacterial cell wall Tetracycline and the Gram negative barrier The spread of antibiotic resistance

Immunology and Disease: The Immune ResponseImmunology

Topic overview Natural barriers to infection Inflammation, clotting and phagocytosis Antigens, B lymphocytes and the humoral response Cell-mediated immunity and T lymphocytes Active and passive immunity Vaccination programmes and their ethics

Human Musculoskeletal Anatomy: Skeletal TissuesMusculoskeletal

Topic overview Cartilage and its three types The composition and functions of compact bone Bone remodelling and the Haversian system Rickets, osteomalacia, osteoporosis and brittle bone disease The structure of skeletal muscle The sliding filament theory Fast and slow twitch fibres, and energy for contraction

Human Musculoskeletal Anatomy: Structure and Function of the SkeletonMusculoskeletal

Topic overview The axial and appendicular skeleton The girdles and the bones of the limbs The vertebral column and its vertebrae Fractures and postural deformities The functions of the skeleton

Human Musculoskeletal Anatomy: JointsMusculoskeletal

Topic overview Types of joint in the human body The structure of a synovial joint Osteoarthritis and rheumatoid arthritis Joints as levers Antagonistic muscle action in the forelimb

Neurobiology and Behaviour: The BrainNeurobiology

Topic overview The structure of the human brain The functions of the cerebrum, cerebellum and medulla oblongata The hypothalamus and the control of the endocrine system The sympathetic and parasympathetic nervous systems Sensory and motor areas, the homunculus and language

Neurobiology and Behaviour: NeuroscienceNeurobiology

Topic overview Non-invasive study of the brain: CT and MRI Functional imaging: fMRI, PET and EEG Brain development and critical periods Neuroplasticity Gene expression, brain development and mental illness

Neurobiology and Behaviour: BehaviourNeurobiology

Topic overview Innate behaviour: escape reflexes, kineses and taxes Habituation and imprinting Classical and operant conditioning Primate societies and the costs and benefits of group living Insect castes, dominance hierarchies, territory and courtship

Importance of ATP

5 subtopics
15 things to know
Component 1

What Importance of ATP covers

Importance of ATP is examined in Component 1, Energy for Life. It covers ATP synthetase and the synthesis of ATP, chemiosmosis and the electrochemical gradient, proton pumps and the electron transport chain, membranes of mitochondria and chloroplasts compared and investigating dehydrogenase activity.

Where it is assessed

Component 1

Energy for Life, whose Core Concepts are also assessed in the other two papers.

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The 5 subtopics

Each one has its own page.

What you need to know

Answer each one before you reveal it.

Your recallNot started

Key term

Why is a proton gradient electrical as well as chemical?

Key term

Describe how yeast and methylene blue are used to investigate dehydrogenase activity.

Key term

Describe how DCPIP is used with isolated chloroplasts, and state what the result shows.

Key term

Where in a mitochondrion is ATP synthetase found?

Key term

How are the components of an electron transport chain arranged in the membrane?

Key term

Which membrane of a mitochondrion carries the machinery for ATP synthesis?

Key term

How does ATP synthetase appear in an electron micrograph of a mitochondrion?

Key term

What is an electrochemical gradient?

Key term

In a mitochondrion, what supplies the high-energy electrons that enter the chain?

Key term

Which membrane of a chloroplast carries the machinery for ATP synthesis?

Key term

Describe how ATP synthetase catalyses the formation of ATP.

Key term

In a mitochondrion, where do the protons flow to when they pass through ATP synthetase?

Key term

In a chloroplast, what supplies the high-energy electrons that enter the chain?

Key term

Compare the direction in which protons are pumped in a mitochondrion and in a chloroplast.

Key term

Why can the speed of the colour change be used to measure dehydrogenase activity?

Take Importance of ATP further

The same content, four other ways.

Questions from this topic

Each one with the model answer underneath.

1
Where in a mitochondrion is ATP synthetase found?
Model answer
Embedded in the inner membrane, in the cristae.
2
How does ATP synthetase appear in an electron micrograph of a mitochondrion?
Model answer
As stalked particles studding the cristae and projecting into the matrix.
3
Which feature of ATP synthetase allows it to use a proton gradient?
Model answer
It contains a channel through the membrane, and this is the only route by which protons can cross an otherwise impermeable membrane.
4
Describe how ATP synthetase catalyses the formation of ATP.
Model answer
Protons flow down the electrochemical gradient through its channel, and the energy released as they pass alters the shape of the enzyme so that it joins ADP to an inorganic phosphate group.
5
State the reaction catalysed by ATP synthetase.
Model answer
ADP combines with an inorganic phosphate group to give ATP and water.
6
Define oxidative phosphorylation.
Model answer
The synthesis of ATP using energy released as electrons pass along the electron transport chain in respiration, with oxygen as the final electron acceptor.

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Importance of ATP revision questions

What does Importance of ATP cover in Eduqas A-Level Biology?: 5 subtopics: atp synthetase and the synthesis of atp, chemiosmosis and the electrochemical gradient, proton pumps and the electron transport chain, membranes of mitochondria and chloroplasts compared and investigating dehydrogenase activity. Each one has its own page with questions and model answers.
How is Importance of ATP organised?: By the Eduqas A400QS specification, subtopic by subtopic, so it matches the order you are taught it in.

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