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Acid-Base Equilibria

Buffers in blood, and the core practical

  • 3 things to know
  • Papers 1 and 3

Overview

How the carbonic acid and hydrogencarbonate buffer holds blood near pH 7.4, Core Practical 9 finding Kₐ from the pH of a weak acid of known concentration, ways of measuring pH, the role of indicators, and decimal places, calculator functions and logarithms in pH calculations.

What you need to know

Answer each one before you reveal it.

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Numbers

At what pH is blood normally maintained?

Key term

What are the products when carbonic acid in blood reacts with added hydroxide ions?

Key term

In the blood buffer system, which species acts as the weak acid and which acts as the conjugate base?

Questions from this subtopic

Each one with the model answer underneath.

  1. 1

    At what pH is blood normally maintained?

    Model answer

    Approximately pH 7.4.

  2. 2

    In the blood buffer system, which species acts as the weak acid and which acts as the conjugate base?

    Model answer

    Carbonic acid (H₂CO₃) acts as the weak acid; hydrogencarbonate ions (HCO₃⁻) act as the conjugate base.

  3. 3

    Explain how the blood buffer system responds when hydrogen ions are added.

    Model answer

    Hydrogencarbonate ions react with H⁺ to form carbonic acid, which then decomposes to carbon dioxide and water.

  4. 4

    Describe the reaction that occurs in blood when hydroxide ions are added, involving the carbonic acid buffer system.

    Model answer

    Carbonic acid reacts with the hydroxide ions to form hydrogencarbonate ions and water, neutralising the added base

  5. 5

    What are the products when carbonic acid in blood reacts with added hydroxide ions?

    Model answer

    Hydrogencarbonate ions (HCO₃⁻) and water

  6. 6

    What is the purpose of Core Practical 9?

    Model answer

    To obtain Kₐ: make up a weak acid at a concentration you know, then measure its pH.

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