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Human Impact on Biodiversity

Biological oxygen demand and reducing aquatic pollution

  • 3 things to know
  • Unit AS 2

Overview

Biological oxygen demand, why the sample must be incubated in complete darkness and why the sample bottle must be filled completely and sealed.

What you need to know

Answer each one before you reveal it.

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Key term

Describe how the biological oxygen demand of a water sample is measured.

Key term

Explain why a sample for measuring biological oxygen demand must be incubated in complete darkness

Key term

Explain why the bottle for a biological oxygen demand sample must be filled completely and sealed

Questions from this subtopic

Each one with the model answer underneath.

  1. 1

    Define biological oxygen demand.

    Model answer

    Biological oxygen demand is the mass of dissolved oxygen consumed by the micro-organisms in a sample of water over a stated period at a stated temperature.

  2. 2

    In what units is biological oxygen demand normally expressed?

    Model answer

    It is normally expressed in mg dm⁻³ of oxygen.

  3. 3

    What does a high biological oxygen demand tell you about a sample of water?

    Model answer

    It shows that the water contains a large amount of organic matter supporting a large population of decomposer micro-organisms, so the water is heavily polluted.

  4. 4

    Describe how the biological oxygen demand of a water sample is measured.

    Model answer

    The dissolved oxygen concentration is measured, an identical sealed sample is incubated in the dark for five days at 20 °C, and the oxygen is measured again, the fall being the biological oxygen demand.

  5. 5

    Explain why the sample must be incubated in complete darkness.

    Model answer

    In the light any algae in the sample would photosynthesise and release oxygen, which would mask the oxygen used by the micro-organisms.

  6. 6

    Explain why the sample bottle must be filled completely and sealed.

    Model answer

    Any air space would allow oxygen to dissolve into the water during the incubation, so the fall in oxygen would be underestimated.

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