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Photosynthesis

Photolysis of water, reduced NADP and redox indicators

  • 3 things to know
  • Unit A2 2

Overview

Photolysis of water, fate of the oxygen produced in the light-dependent stage and why reduced NADP is needed by the light-independent stage.

What you need to know

Answer each one before you reveal it.

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Key term

Explain why the medium used to isolate chloroplasts is kept cold, isotonic and buffered.

Key term

Define photolysis of water.

Key term

Write an equation for the dissociation of water in the thylakoid.

Questions from this subtopic

Each one with the model answer underneath.

  1. 1

    Define photolysis of water.

    Model answer

    Photolysis is the splitting of water inside the thylakoid using light energy absorbed by the photosystems.

  2. 2

    Write an equation for the dissociation of water in the thylakoid.

    Model answer

    Water dissociates as H₂O ⇌ H⁺ + OH⁻.

  3. 3

    What happens to the hydrogen ions produced when water dissociates?

    Model answer

    The hydrogen ions combine with NADP and the electrons arriving from photosystem I, producing reduced NADP.

  4. 4

    What happens to the hydroxide ions produced when water dissociates?

    Model answer

    The hydroxide ions give up electrons to photosystem II, and the residual groups react together to form water and oxygen.

  5. 5

    Write the equation showing how oxygen is formed from hydroxide ions.

    Model answer

    The reaction is 4OH⁻ → 2H₂O + O₂ + 4e⁻.

  6. 6

    From which molecule does the oxygen released by a photosynthesising plant originate?

    Model answer

    All the oxygen released comes from water that has been split, not from carbon dioxide.

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